Nowadays both part-time as well as regular employees are working in many organizations of Bangladesh. Though many studies have been conducted to know the motivation status of regular employees but no study is found that addressed motivations status of both regular and part-time employees of Bangladesh. Thus, this study is conducted on 300 regular and part-time employees of Bangladesh to know the most motivating factors of both regular and part-time employees. Respondents were asked to identify and rank ten motivation factors in the order of their magnitude. The findings reveal that good salary, job security, good working condition and interesting work are the most important motivating factors for the regular employees, whereas; flexibility of work, interesting work, good salary and good work environment are the most important motivating factor for part-time employees, because most of the part-time employees working in different organizations are either students or regular employees of other organizations. The findings of the study can be useful for managers and human resource (HR) professionals for motivating regular employees as well as part-time employees and thereby achieving the goals of organization more effectively and efficiently.